Journal article
Intimal macrophages develop from circulating monocytes during vasculitis
AT Stock, S Parsons, VJ Sharma, F James, G Starkey, R D'Costa, CL Gordon, IP Wicks
Clinical and Translational Immunology | WILEY | Published : 2022
DOI: 10.1002/cti2.1412
Abstract
Objective: Vasculitis is characterised by inflammation of the blood vessels. While all layers of the vessel can be affected, inflammation within the intimal layer can trigger thrombosis and arterial occlusion and is therefore of particular clinical concern. Given this pathological role, we have examined how intimal inflammation develops by exploring which (and how) macrophages come to populate this normally immune-privileged site during vasculitis. Methods: We have addressed this question for Kawasaki disease (KD), which is a type of vasculitis in children that typically involves the coronary arteries. We used confocal microscopy and flow cytometry to characterise the macrophages that popula..
View full abstractGrants
Awarded by National Health and Medical Research Council
Funding Acknowledgements
We thank Tom Kitson, Lauren Wilkins, Jacinta Hansen, Huon Wong and Damian D'Silva (WEHI, Melbourne, Australia) for outstanding technical assistance and Drs Andrew Lew and Yifan Zhan (WEHI, Melbourne, Australia) for the provision of mice. This research has been conducted using samples and data from the Australian Donation and Transplantation Biobank (ADTB). We acknowledge the following ADTB collaborators: Lisa Davies, Effie Mouhtouris, Robert Jones, Bao Zhong Wang, M. Lindsay Grayson, Helen Opdam, Jai Raman, Angela Vago and The Austin Transplant Perfusionist Group. We gratefully acknowledge the generosity of the organ donor families for providing invaluable tissue samples. This work was supported by the Australian National Health and Medical Research Council (NHMRC) Program Grant 1113577 and Clinical Practitioner Fellowship 1154235 (to IPW), the Reid Charitable Trusts and NHMRC Early Career Fellowship GNT 1160963 (CLG). This study was made possible through Victorian State Government Operational Infrastructure Support and the Australian Government National Health and Medical Research Council Independent Research Institute Infrastructure Support scheme.